Cells react in different ways both metabolically and morphologically depending on the environmental factors maintaining and interacting with them.
In tissue culture, faithfully reproducing the in vivo cellular environment is vital for meaningful analysis of both cell metabolism and cell function.
Cells in vivo experience oxygen concentrations in the range of 5 – 80 mmHg (approx. 0.5 – 10% oxygen), depending on the tissue type. Yet in vitro cell biology is typically performed in incubators in which cells are exposed to atmospheric levels of oxygen (21%), a 'hyperoxic' state for most cell types. In other words, the oxygen concentration typically encountered by cells in traditional incubators is 2 - 20 times that experienced in living tissues!
Maintaining cells in such an oxygen-rich environment can have profound implications on cell metabolism, as illustrated by the large body of scientific evidence surrounding the hypoxia-inducible factor (HIF) pathways.
The HypoxyLab™ reproduces physiological conditions in cell-based research by delivering a contamination-free tissue culture environment that offers precise control of oxygen, CO₂, temperature, and humidity. And it does so in a remarkably compact and ergonomic form-factor that offers unmatched ease-of-use and economical gas consumption.
Meanwhile, it is the partial pressure of oxygen, not merely the proportion of oxygen in the environment (% oxygen) that cells in culture actually 'see'. HypoxyLab adopts this fundamental scientific principle and controls the chamber environment using the partial pressure of oxygen (pO₂) expressed in units of mmHg or kPa.
Since the partial pressure of oxygen varies not only with oxygen concentration but also with altitude and prevailing atmospheric pressure, this scientifically rigorous approach substantially enhances HypoxyLab's accuracy relative to other hypoxia chambers and workstations that rely on % oxygen concentration control alone.
Furthermore, this approach addresses a secondary shortcoming affecting traditional hypoxic/physoxic workstations in use today: reproducibility. By intrinsically correcting for both altitude and atmospheric pressure, data generated by one HypoxyLab user can always be directly compared to data generated by another HypoxyLab user, wherever they may be situated.
11. Rationale